Shanghai spectrophotometer has become a conventional instrument in modern molecular biology laboratory.

Release time：2019-03-19

Shanghai spectrophotometer has become a conventional instrument in modern molecular biology laboratory. It is often used to quantify nucleic acid, protein and bacterial growth concentration. Shanghai spectrophotometer is mainly composed of light source, monochromator, sample room, detector, signal processor and display and storage system.



Several points for attention in the use of Shanghai spectrophotometer:



1. When the equipment is stored, some desiccants should be put in the place where it is stored to avoid dampness affecting the use effect.



2. Pay attention to the cleanliness of the stored dishes. When cleaning, some parts are not allowed to be wiped directly by hand. Pay attention to the use of special cleaning tools. When picking up the colorimetric dish, the finger can only hold the ground glass surface of the colorimetric dish, but can not touch the optical surface of the colorimetric dish.



Third, try not to drop any liquid on the instrument. Once this phenomenon occurs, we should use special cleaning tools to wipe it in * time. Colorimetric dishes can not be washed with alkali solution or strong oxidizing detergent, nor can they be washed with a brush. The water or solution attached to the outer wall of the colorimetric dish should be absorbed by a mirror paper or a thin and soft absorbent paper. Do not wipe it to avoid damaging its optical surface.



4. After use, one part and one part should be cleaned, especially the color dishes. The dishes should be cleaned with special cleaning liquid, and then dried before being put into storage equipment. In order to prevent the phototube from fatigue, the sample chamber cover must be opened to cut off the light path so as to prolong the service life of the phototube.



Spectrophotometer problem handling:



1. If the instrument cannot be initialized, shut down and restart.



2. If the absorption value is abnormal, check in turn: whether the wavelength setting is correct (re-adjust the wavelength and re-zero), whether the measurement is zero (such as misoperation, re-zero), whether the colorimetric dish is wrong (quartz colorimetric dish is used to determine the ultraviolet band), whether the sample preparation is wrong (if there is a mistake, re-prepare the sample).



Shanghai spectrophotometer is a precise instrument. Correct installation, use and maintenance play an important role in maintaining the good performance of the instrument and ensuring the accuracy of the test. In order to ensure the accuracy and reliability of test results, spectrophotometers newly manufactured, used and repaired should be checked regularly. It is necessary to adjust the unqualified instruments in time.